Cultrex

sanyo-biomedical

High-performance liquid chromatography under partially denaturing conditions

Identification of copy quantity variation of CAPN10 in Thais with sort 2 diabetes by multiplex PCR and denaturing excessive efficiency liquid chromatography (DHPLC).   Copy quantity variations (CNVs) have been proven to be related to a number of illnesses. They will trigger deviation of genotypes from Hardy-Weinberg Equilibrium (HWE). Genetic case-control affiliation research in Thais […]

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sanyo-biomedical

A novel DHPLC-based procedure for the analysis of COL1A1

Validation of the PCR-dHPLC methodology for fast identification of Candida glabrata phylogenetically associated species in several organic matrices. Since two new species phylogenetically associated to Candida glabrata with barely completely different phenotypes and antifungal susceptibility profiles have been described, it appears to be obligatory from medical standpoint, to develop a fast and correct identification system with […]

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sanyo-biomedical

genes associated with hearing loss in the Japanese population

Growth of a brand new DHPLC assay for genotyping UGT1A (TA)n polymorphism related to Gilbert’s syndrome. BACKGROUND Gilbert’s syndrome is the most typical hereditary dysfunction of bilirubin metabolism. The causative mutation in Caucasians is sort of completely a (TA) dinucleotide insertion within the UGT1A1 promoter. Affected people are homozygous for the variant promoter and have 7 TA […]

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sanyo-biomedical

mutations in gyrB using denaturing high performance liquid chromatography

Detection of mutations in gyrB utilizing denaturing excessive efficiency liquid chromatography (DHPLC) amongst Salmonella enterica serovar Typhi and Paratyphi A. Fluoroquinolone resistance is mediated by mutations within the quinolone-resistance figuring out area (QRDR) of the topoisomerase genes. Denaturing excessive efficiency liquid chromatography (DHPLC) was evaluated for detection of clinically essential mutations in gyrB amongst Salmonella. […]

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