High-performance liquid chromatography under partially denaturing conditions

sanyo-biomedical

Identification of copy quantity variation of CAPN10 in Thais with sort 2 diabetes by multiplex PCR and denaturing excessive efficiency liquid chromatography (DHPLC).

 

Copy quantity variations (CNVs) have been proven to be related to a number of illnesses. They will trigger deviation of genotypes from Hardy-Weinberg Equilibrium (HWE). Genetic case-control affiliation research in Thais revealed that genotype distribution of CAPN10 Indel19 was deviated from HWE after correction of genotyping error. Due to this fact, we intention to establish CNVs inside CAPN10 Indel19 area. The semi-quantitative denaturating excessive efficiency liquid chromatography (DHPLC) technique was used to detect CNVs within the area of CAPN10 Indel19 marker in cohort of 305 sufferers with sort 2 diabetes and 250 management topics with out diabetes. CNVs within the area of CAPN10 Indel19 was efficiently detected by DHPLC.

After correction of genotype calling primarily based on the standing of recognized CNVs, CAPN10 Indel19 genotypes had been well-fitted for HWE (p>0.05). Nevertheless, we didn’t discover affiliation between CNV genotypes and danger of sort 2 diabetes in our inhabitants. CNVs in CAPN10 have been recognized in Thais. These CNVs result in deviation from HWE of CAPN10 Indel19 genotypes. After excluding recognized CNVs from the evaluation, CAPN10 Indel19 was related to sort 2 diabetes. The data obtained from our examine could be useful for genotyping accuracies of SNPs residing within the CNVs area.

DHPLC/SURVEYOR nuclease: a delicate, speedy and reasonably priced technique to research BRCA1 and BRCA2 mutations in breast most cancers households.

Hereditary breast most cancers accounts for about 10% of all breast cancers and BRCA1 and BRCA2 genes have been recognized as validated susceptibility genes for this pathology. Testing for BRCA gene mutations is normally primarily based on a pre-screening method, such because the partial denaturation DHPLC technique, and capillary direct sequencing. Nevertheless, this method is time consuming as a result of giant measurement of BRCA1 and BRCA2 genes. Not too long ago, a brand new low price and time saving DHPLC protocol has been developed to research gene mutations through the use of SURVEYOR(®) Nuclease digestion and DHPLC evaluation. A subset of 90 sufferers, enrolled within the Genetic Counseling Program of the Nationwide Most cancers Centre of Bari (Italy), was carried out to validate this method.

Earlier retrospective evaluation confirmed that 9/90 sufferers (10%) had been mutated in BRCA1 and BRCA2 genes and these knowledge had been confirmed by the current method. DNA samples underwent landing PCR and, subsequently, SURVEYOR(®) nuclease digestion. BRCA1 and BRCA2 amplicons had been divided into teams relying on amplicon measurement to permit multiamplicon digestion. The product of this response had been analyzed on Transgenomic WAVE Nucleic Acid Excessive Sensitivity Fragment Evaluation System. The operator who carried out the DHPLC surveyor method didn’t know the sequencing outcomes at the moment. The SURVEYOR(®) Nuclease DHPLC method was in a position to detect all alterations with a sensitivity of 95%. Moreover, to be able to save time and reagents, a multiamplicon setting preparation was validated.

sanyo-biomedical
sanyo-biomedical

Recombinant Human Myosin-1 (MYH1) , partial

MBS964626-002mgYeast 0.02mg(Yeast)
EUR 880

Recombinant Human Myosin-1 (MYH1) , partial

MBS964626-01mgEColi 0.1mg(E-Coli)
EUR 855

Recombinant Human Myosin-1 (MYH1) , partial

MBS964626-01mgYeast 0.1mg(Yeast)
EUR 1035

Recombinant Pig Myosin-1 (MYH1), partial

MBS1462893-INQUIRE INQUIRE Ask for price

Recombinant Mouse Myosin-1 (Myh1) , partial

MBS1477688-INQUIRE INQUIRE Ask for price

Recombinant Horse Myosin-1 (MYH1), partial

MBS1466320-INQUIRE INQUIRE Ask for price

Recombinant Bovine Myosin-1 (MYH1), partial

CSB-EP858986BO 10533 mg Ask for price

Recombinant Bovine Myosin-1 (MYH1) , partial

MBS1458010-INQUIRE INQUIRE Ask for price

Recombinant Human Myosin-14 (MYH14) , partial

MBS1294964-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1645

Recombinant Human Myosin-14 (MYH14) , partial

MBS1294964-002mgEColi 0.02mg(E-Coli)
EUR 1390

Recombinant Human Myosin-14 (MYH14) , partial

MBS1294964-002mgYeast 0.02mg(Yeast)
EUR 1405

Recombinant Human Myosin-14 (MYH14) , partial

MBS1294964-01mgEColi 0.1mg(E-Coli)
EUR 1675

Recombinant Human Myosin-14 (MYH14) , partial

MBS1294964-01mgYeast 0.1mg(Yeast)
EUR 1650

Recombinant Human Myosin-13 (MYH13) , partial

MBS1301859-INQUIRE INQUIRE Ask for price

Recombinant Human Myosin-11 (MYH11) , partial

MBS718804-005mgMammalianCell 0.05mg(Mammalian-Cell)
EUR 1240

Recombinant Human Myosin-11 (MYH11) , partial

MBS718804-05mgEColi 0.5mg(E-Coli)
EUR 1020

Recombinant Human Myosin-11 (MYH11) , partial

MBS718804-05mgYeast 0.5mg(Yeast)
EUR 1240

Recombinant Human Myosin-11 (MYH11) , partial

MBS718804-1mgEColi 1mg(E-Coli)
EUR 1380

Recombinant Human Myosin-11 (MYH11) , partial

MBS718804-1mgYeast 1mg(Yeast)
EUR 1890

Recombinant Human Myosin-15 (MYH15) , partial

MBS1378539-INQUIRE INQUIRE Ask for price

Recombinant Human Myosin-10 (MYH10), partial

MBS955851-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1920

Recombinant Human Myosin-10 (MYH10), partial

MBS955851-01mgBaculovirus 0.1mg(Baculovirus)
EUR 2330

Recombinant Human Myosin-10 (MYH10), partial

MBS955851-05mgEColi 0.5mg(E-Coli)
EUR 2095

Recombinant Human Myosin-10 (MYH10), partial

MBS955851-05mgYeast 0.5mg(Yeast)
EUR 2210

Recombinant Human Myosin-10 (MYH10), partial

MBS955851-1mgEColi 1mg(E-Coli)
EUR 2545

Recombinant Human Myosin-6 (MYH6), partial

CSB-YP015299HU 5861 mg Ask for price

Recombinant Human Myosin-9(MYH9) ,partial

AP71716 1mg
EUR 1978

Recombinant Human Myosin-6(MYH6),partial

AP72695 each Ask for price

Recombinant Human Myosin-6 (MYH6), partial

CSB-EP015299HU 6810 mg Ask for price

Recombinant Human Myosin-6 (MYH6), partial

CSB-EP015299HUa0 9932 mg Ask for price

Recombinant Human Myosin-7 (MYH7), partial

CSB-EP015300HU 9938 mg Ask for price

Recombinant Human Myosin-9 (MYH9), partial

CSB-EP015303HUe0 1758 mg Ask for price

Recombinant Human Myosin-6 (MYH6), partial

CSB-BP015299HU 10121 mg Ask for price

Recombinant Human Myosin-7 (MYH7), partial

CSB-BP015300HU 9781 mg Ask for price

Recombinant Human Myosin-2 (MYH2) , partial

MBS1302144-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1070

Recombinant Human Myosin-2 (MYH2) , partial

MBS1302144-005mgEColi 0.05mg(E-Coli)
EUR 745

Recombinant Human Myosin-2 (MYH2) , partial

MBS1302144-005mgYeast 0.05mg(Yeast)
EUR 905

Recombinant Human Myosin-2 (MYH2) , partial

MBS1302144-02mgEColi 0.2mg(E-Coli)
EUR 990

Recombinant Human Myosin-2 (MYH2) , partial

MBS1302144-05mgEColi 0.5mg(E-Coli)
EUR 1090

Recombinant Human Myosin-9 (MYH9) ,partial

RPC20174-100ug 100ug
EUR 460.9

Recombinant Human Myosin-9 (MYH9) ,partial

RPC20174-1mg 1mg
EUR 2237.8

Recombinant Human Myosin-9 (MYH9) ,partial

RPC20174-20ug 20ug
EUR 258.7

Recombinant Human Myosin-6 (MYH6) ,partial

RPC20235-100ug 100ug
EUR 573.1

Recombinant Human Myosin-6 (MYH6) ,partial

RPC20235-20ug 20ug
EUR 288.8

Recombinant Human Myosin-8 (MYH8) , partial

MBS717528-INQUIRE INQUIRE Ask for price

Recombinant Human Myosin-9 (MYH9) , partial

MBS718871-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Human Myosin-9 (MYH9) , partial

MBS718871-002mgEColi 0.02mg(E-Coli)
EUR 750

Recombinant Human Myosin-9 (MYH9) , partial

MBS718871-002mgYeast 0.02mg(Yeast)
EUR 910

Recombinant Human Myosin-9 (MYH9) , partial

MBS718871-01mgEColi 0.1mg(E-Coli)
EUR 900

Recombinant Human Myosin-9 (MYH9) , partial

MBS718871-01mgYeast 0.1mg(Yeast)
EUR 1065

Recombinant Human Myosin-6 (MYH6) , partial

MBS962226-002mgEColi 0.02mg(E-Coli)
EUR 255

Recombinant Human Myosin-6 (MYH6) , partial

MBS962226-002mgYeast 0.02mg(Yeast)
EUR 270

Recombinant Human Myosin-6 (MYH6) , partial

MBS962226-01mgEColi 0.1mg(E-Coli)
EUR 405

Recombinant Human Myosin-6 (MYH6) , partial

MBS962226-01mgYeast 0.1mg(Yeast)
EUR 450

Recombinant Human Myosin-6 (MYH6) , partial

MBS962226-05mgYeast 0.5mg(Yeast)
EUR 1065

Recombinant Human Myosin-3 (MYH3) , partial

MBS949055-INQUIRE INQUIRE Ask for price

Recombinant Human Myosin-7 (MYH7) , partial

MBS965074-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1030

Recombinant Human Myosin-7 (MYH7) , partial

MBS965074-002mgEColi 0.02mg(E-Coli)
EUR 610

Recombinant Human Myosin-7 (MYH7) , partial

MBS965074-002mgYeast 0.02mg(Yeast)
EUR 785

Recombinant Human Myosin-7 (MYH7) , partial

MBS965074-01mgEColi 0.1mg(E-Coli)
EUR 705

Recombinant Human Myosin-7 (MYH7) , partial

MBS965074-01mgYeast 0.1mg(Yeast)
EUR 920

Recombinant Human Myosin-7B (MYH7B) , partial

MBS1149986-INQUIRE INQUIRE Ask for price

Recombinant Rat Myosin-10 (Myh10) , partial

MBS1414792-INQUIRE INQUIRE Ask for price

Recombinant Rat Myosin-11 (Myh11) , partial

MBS1378164-INQUIRE INQUIRE Ask for price

Recombinant Rat Myosin-9 (Myh9), partial

CSB-EP717285RA 6891 mg Ask for price

Recombinant Rat Myosin-6 (Myh6) , partial

MBS1126348-INQUIRE INQUIRE Ask for price

Recombinant Rat Myosin-7 (Myh7) , partial

MBS1092149-INQUIRE INQUIRE Ask for price

Recombinant Rat Myosin-9 (Myh9) , partial

MBS1288820-002mgEColi 0.02mg(E-Coli)
EUR 375

Recombinant Rat Myosin-9 (Myh9) , partial

MBS1288820-01mgEColi 0.1mg(E-Coli)
EUR 635

Recombinant Rat Myosin-9 (Myh9) , partial

MBS1288820-1mgEColi 1mg(E-Coli)
EUR 1925

Recombinant Rat Myosin-9 (Myh9) , partial

MBS1288820-5x1mgEColi 5x1mg(E-Coli)
EUR 8405

Recombinant Dog Myosin-9 (MYH9), partial

MBS1320195-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1290

Recombinant Dog Myosin-9 (MYH9), partial

MBS1320195-002mgEColi 0.02mg(E-Coli)
EUR 970

Recombinant Dog Myosin-9 (MYH9), partial

MBS1320195-002mgYeast 0.02mg(Yeast)
EUR 1080

Recombinant Dog Myosin-9 (MYH9), partial

MBS1320195-01mgEColi 0.1mg(E-Coli)
EUR 1130

Recombinant Dog Myosin-9 (MYH9), partial

MBS1320195-01mgYeast 0.1mg(Yeast)
EUR 1230

Recombinant Pig Myosin-4 (MYH4), partial

MBS1354999-INQUIRE INQUIRE Ask for price

Recombinant Rat Myosin-4 (Myh4) , partial

MBS1421500-INQUIRE INQUIRE Ask for price

Recombinant Pig Myosin-2 (MYH2), partial

MBS1383161-INQUIRE INQUIRE Ask for price

Recombinant Rat Myosin-3 (Myh3) , partial

MBS956257-INQUIRE INQUIRE Ask for price

Recombinant Dog Myosin-7 (MYH7), partial

MBS953119-INQUIRE INQUIRE Ask for price

Recombinant Pig Myosin-7 (MYH7), partial

MBS966458-INQUIRE INQUIRE Ask for price

Recombinant Mouse Myosin-11 (Myh11) , partial

MBS1006374-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1075

Recombinant Mouse Myosin-11 (Myh11) , partial

MBS1006374-002mgEColi 0.02mg(E-Coli)
EUR 665

Recombinant Mouse Myosin-11 (Myh11) , partial

MBS1006374-002mgYeast 0.02mg(Yeast)
EUR 830

Recombinant Mouse Myosin-11 (Myh11) , partial

MBS1006374-01mgEColi 0.1mg(E-Coli)
EUR 775

Recombinant Mouse Myosin-11 (Myh11) , partial

MBS1006374-01mgYeast 0.1mg(Yeast)
EUR 970

Recombinant Mouse Myosin-14 (Myh14) , partial

MBS1284681-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1645

Recombinant Mouse Myosin-14 (Myh14) , partial

MBS1284681-002mgEColi 0.02mg(E-Coli)
EUR 1395

Recombinant Mouse Myosin-14 (Myh14) , partial

MBS1284681-002mgYeast 0.02mg(Yeast)
EUR 1405

Recombinant Mouse Myosin-14 (Myh14) , partial

MBS1284681-01mgEColi 0.1mg(E-Coli)
EUR 1685

Recombinant Mouse Myosin-14 (Myh14) , partial

MBS1284681-01mgYeast 0.1mg(Yeast)
EUR 1655

Recombinant Mouse Myosin-10 (Myh10) , partial

MBS1413163-INQUIRE INQUIRE Ask for price

Recombinant Bovine Myosin-10 (MYH10) , partial

MBS1393245-INQUIRE INQUIRE Ask for price

Recombinant Rabbit Myosin-11 (MYH11), partial

MBS948611-INQUIRE INQUIRE Ask for price

Recombinant Mouse Myosin-9 (Myh9) , partial

MBS1281774-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1125

Recombinant Mouse Myosin-9 (Myh9) , partial

MBS1281774-005mgEColi 0.05mg(E-Coli)
EUR 750

Recombinant Mouse Myosin-9 (Myh9) , partial

MBS1281774-005mgYeast 0.05mg(Yeast)
EUR 910

Recombinant Mouse Myosin-9 (Myh9) , partial

MBS1281774-02mgEColi 0.2mg(E-Coli)
EUR 1000

Recombinant Mouse Myosin-9 (Myh9) , partial

MBS1281774-05mgEColi 0.5mg(E-Coli)
EUR 1100

Recombinant Horse Myosin-7 (MYH7), partial

MBS1284539-INQUIRE INQUIRE Ask for price

Recombinant Mouse Myosin-4 (Myh4) , partial

MBS1473570-INQUIRE INQUIRE Ask for price

Recombinant Horse Myosin-2 (MYH2), partial

MBS1430254-INQUIRE INQUIRE Ask for price

Recombinant Mouse Myosin-7 (Myh7) , partial

MBS1336124-INQUIRE INQUIRE Ask for price

Recombinant Mouse Myosin-3 (Myh3) , partial

MBS955980-INQUIRE INQUIRE Ask for price

Recombinant Mouse Myosin-6 (Myh6) , partial

MBS952668-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1100

Recombinant Mouse Myosin-6 (Myh6) , partial

MBS952668-005mgEColi 0.05mg(E-Coli)
EUR 695

Recombinant Mouse Myosin-6 (Myh6) , partial

MBS952668-005mgYeast 0.05mg(Yeast)
EUR 855

Recombinant Mouse Myosin-6 (Myh6) , partial

MBS952668-02mgEColi 0.2mg(E-Coli)
EUR 905

Recombinant Mouse Myosin-6 (Myh6) , partial

MBS952668-05mgEColi 0.5mg(E-Coli)
EUR 995

Recombinant Mouse Myosin-8 (Myh8) , partial

MBS965620-INQUIRE INQUIRE Ask for price

Recombinant Chicken Myosin-11 (MYH11), partial

MBS961625-INQUIRE INQUIRE Ask for price

Recombinant Rabbit Myosin-4 (MYH4), partial

MBS1295147-INQUIRE INQUIRE Ask for price

Recombinant Bovine Myosin-2 (MYH2) , partial

MBS1342661-INQUIRE INQUIRE Ask for price

Recombinant Bovine Myosin-7 (MYH7) , partial

MBS1398011-INQUIRE INQUIRE Ask for price

Recombinant Rabbit Myosin-7 (MYH7), partial

MBS965416-INQUIRE INQUIRE Ask for price

Recombinant Chicken Myosin-3 (MYH3), partial

MBS950700-INQUIRE INQUIRE Ask for price

Recombinant Chicken Myosin-9 (MYH9), partial

MBS966607-INQUIRE INQUIRE Ask for price

Recombinant Mouse Myosin-7B (Myh7b) , partial

MBS1130490-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1555

Recombinant Mouse Myosin-7B (Myh7b) , partial

MBS1130490-002mgEColi 0.02mg(E-Coli)
EUR 1275

Recombinant Mouse Myosin-7B (Myh7b) , partial

MBS1130490-002mgYeast 0.02mg(Yeast)
EUR 1315

Recombinant Mouse Myosin-7B (Myh7b) , partial

MBS1130490-01mgEColi 0.1mg(E-Coli)
EUR 1535

Recombinant Mouse Myosin-7B (Myh7b) , partial

MBS1130490-01mgYeast 0.1mg(Yeast)
EUR 1545

Recombinant Mesocricetus auratus Myosin-6 (MYH6), partial

MBS1166544-INQUIRE INQUIRE Ask for price

Recombinant Mesocricetus auratus Myosin-7 (MYH7), partial

MBS1124826-INQUIRE INQUIRE Ask for price

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU56815-100ug 100ug
EUR 470.4

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU56815-1mg 1mg
EUR 2184

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU56815-50ug 50ug
EUR 385

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU52400-100ug 100ug
EUR 524.7

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU52400-1mg 1mg
EUR 2325.7

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU52400-50ug 50ug
EUR 421.3

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU42866-100ug 100ug
EUR 554.4

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU42866-1mg 1mg
EUR 2457

Recombinant Myosin Heavy Chain 1 (MYH1)

RPU42866-50ug 50ug
EUR 445.5

Recombinant Myosin Heavy Chain 1 (MYH1)

RPA754Hu01 10ug
EUR 170

Recombinant Myosin Heavy Chain 1 (MYH1)

RPA754Mu01 10ug
EUR 180

Recombinant Human Myosin-IIIa (MYO3A) , partial

MBS1460916-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1150

Recombinant Human Myosin-IIIa (MYO3A) , partial

MBS1460916-005mgMammalianCell 0.05mg(Mammalian-Cell)
EUR 1405

Recombinant Human Myosin-IIIa (MYO3A) , partial

MBS1460916-05mgEColi 0.5mg(E-Coli)
EUR 1150

Recombinant Human Myosin-IIIa (MYO3A) , partial

MBS1460916-05mgYeast 0.5mg(Yeast)
EUR 1405

Recombinant Human Myosin-IIIa (MYO3A) , partial

MBS1460916-1mgEColi 1mg(E-Coli)
EUR 1745

Recombinant Human Myosin-IIIb (MYO3B) , partial

MBS1430174-INQUIRE INQUIRE Ask for price

Human Myosin-1 (MYH1) Protein

abx168162-1ml 1 ml
EUR 225

Human Myosin 1(MYH1) ELISA kit

E01A5966 96T
EUR 700
Description: ELISA

Human Myosin 1(MYH1) ELISA kit

E01M0413-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1(MYH1) ELISA kit

E01M0413-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1(MYH1) ELISA kit

E01M0413-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1 (MYH1) ELISA Kit

MBS7239025-10x96StripWells 10x96-Strip-Wells
EUR 5685

Human Myosin 1 (MYH1) ELISA Kit

MBS7239025-48StripWells 48-Strip-Wells
EUR 485

Human Myosin 1 (MYH1) ELISA Kit

MBS7239025-5x96StripWells 5x96-Strip-Wells
EUR 3020

Human Myosin 1 (MYH1) ELISA Kit

MBS7239025-96StripWells 96-Strip-Wells
EUR 690

Recombinant Human Unconventional myosin-X (MYO10), partial

CSB-EP884628HU 11647 mg Ask for price

Recombinant Human Unconventional myosin-VI (MYO6) , partial

MBS1479859-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventionnal myosin-X (MYO10) , partial

MBS1376326-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-If (MYO1F) , partial

MBS1199586-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-Ic (MYO1C) , partial

MBS1113756-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-Ib (MYO1B) , partial

MBS1245754-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1580

Recombinant Human Unconventional myosin-Ib (MYO1B) , partial

MBS1245754-002mgEColi 0.02mg(E-Coli)
EUR 1310

Recombinant Human Unconventional myosin-Ib (MYO1B) , partial

MBS1245754-002mgYeast 0.02mg(Yeast)
EUR 1340

Recombinant Human Unconventional myosin-Ib (MYO1B) , partial

MBS1245754-01mgEColi 0.1mg(E-Coli)
EUR 1585

Recombinant Human Unconventional myosin-Ib (MYO1B) , partial

MBS1245754-01mgYeast 0.1mg(Yeast)
EUR 1580

Recombinant Human Unconventional myosin-Id (MYO1D) , partial

MBS1264568-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-Ig (MYO1G) , partial

MBS1041865-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-Vb (MYO5B) , partial

MBS1473407-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-Ie (MYO1E) , partial

MBS1391020-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-Vc (MYO5C) , partial

MBS1421677-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-Ih (MYO1H) , partial

MBS1459472-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-Ia (MYO1A) , partial

MBS1334206-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-XV (MYO15A) , partial

MBS1294374-INQUIRE INQUIRE Ask for price

Recombinant Human Unconventional myosin-XVI(MYO16), partial

MBS9018947-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1020

Recombinant Human Unconventional myosin-XVI(MYO16), partial

MBS9018947-002mgEColi 0.02mg(E-Coli)
EUR 595

Recombinant Human Unconventional myosin-XVI(MYO16), partial

MBS9018947-002mgYeast 0.02mg(Yeast)
EUR 775

Recombinant Human Unconventional myosin-XVI(MYO16), partial

MBS9018947-01mgEColi 0.1mg(E-Coli)
EUR 690

Recombinant Human Unconventional myosin-XVI(MYO16), partial

MBS9018947-01mgYeast 0.1mg(Yeast)
EUR 905

Recombinant Human Unconventional myosin-XVI (MYO16) , partial

MBS1396668-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1590

Recombinant Human Unconventional myosin-XVI (MYO16) , partial

MBS1396668-002mgEColi 0.02mg(E-Coli)
EUR 1320

Recombinant Human Unconventional myosin-XVI (MYO16) , partial

MBS1396668-002mgYeast 0.02mg(Yeast)
EUR 1350

Recombinant Human Unconventional myosin-XVI (MYO16) , partial

MBS1396668-01mgEColi 0.1mg(E-Coli)
EUR 1595

Recombinant Human Unconventional myosin-XVI (MYO16) , partial

MBS1396668-01mgYeast 0.1mg(Yeast)
EUR 1585

Recombinant Human Unconventional myosin-IXa (MYO9A) , partial

MBS1202620-1mgEColi 1mg(E-Coli)
EUR 1575

Recombinant Human Unconventional myosin-IXa (MYO9A) , partial

MBS1202620-1mgYeast 1mg(Yeast)
EUR 2080

Recombinant Human Unconventional myosin-IXb (MYO9B) , partial

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EUR 1120

Recombinant Human Unconventional myosin-IXb (MYO9B) , partial

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EUR 735

Recombinant Human Unconventional myosin-IXb (MYO9B) , partial

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EUR 895

Recombinant Human Unconventional myosin-IXb (MYO9B) , partial

MBS1287391-01mgEColi 0.1mg(E-Coli)
EUR 880

Recombinant Human Unconventional myosin-IXb (MYO9B) , partial

MBS1287391-01mgYeast 0.1mg(Yeast)
EUR 1050

Excessive-performance liquid chromatography underneath partially denaturing situations (dHPLC) is a quick and cost-effective technique for screening molecular defects: 4 novel mutations present in X-linked power granulomatous illness.

Implementing exact methods in routine prognosis of power granulomatous illness (CGD), which expedite the screening of molecular defects, could also be essential for a fast assumption of affected person prognosis. This examine in contrast the efficacy of single-strand conformation polymorphism evaluation (SSCP) and high-performance liquid chromatography underneath partially denaturing situations (dHPLC) for screening mutations in CGD sufferers. We chosen 10 male CGD sufferers with a medical historical past of extreme recurrent infections and irregular respiratory burst perform. gDNA, mRNA and cDNA samples had been ready by normal strategies. CYBB exons had been amplified by PCR and screened by SSCP or dHPLC. Irregular DNA fragments had been sequenced to disclose the character of the mutations.

The SSCP and dHPLC strategies confirmed DNA abnormalities, respectively, in 55% and 100% of the instances. Sequencing of the irregular DNA samples confirmed mutations in all instances. 4 novel mutations in CYBB had been recognized which had been picked up solely by the dHPLC screening (c.904 insC, c.141+5 g>t, c.553 T>C, and c.665 A>T). This work highlights the relevance of dHPLC, a delicate, quick, dependable and cost-effective technique for screening mutations in CGD, which together with practical assays assessing the phagocyte respiratory burst will contribute to expedite the definitive prognosis of X-linked CGD, direct remedy, genetic counselling and to have a transparent assumption of the prognosis. This technique is very appropriate for creating international locations.

dHPLC effectivity for semi-automated cDNA-AFLP analyses and fragment assortment within the apple scab-resistance gene mannequin

cDNA-AFLP evaluation for transcript profiling has been efficiently utilized to review many plant organic programs, notably plant-microbe interactions. Nevertheless, the separation of cDNA-AFLP fragments by gel electrophoresis is reported to be labor-intensive with solely restricted potential for automation, and the gathering of differential bands for gene identification is much more cumbersome.

On this work, we current using dHPLC (denaturing excessive efficiency liquid chromatography) and automatic DNA fragment assortment utilizing the WAVE(®) System to research and recuperate cDNA-AFLP fragments.

The strategy is efficiently utilized to the Malus-Venturia inaequalis interplay, making it potential to gather 66 completely different transcript-derived fragments for apple genes putatively concerned within the protection response activated by the HcrVf2 resistance gene.

The outcomes, validated by actual time quantitative RT-PCR, had been per the plant-pathogen interplay underneath investigation and this additional helps the suitability of dHPLC for cDNA-AFLP transcript profiling. Options and benefits of this new method are mentioned, evincing that it’s an virtually totally automatable and cost-effective answer for processing giant numbers of samples and accumulating differential genes concerned in different organic processes and non-model crops.

The usage of DHPLC (Denaturing Excessive Efficiency Liquid Chromatography) in II degree screening of the CFTR gene in Prenatal Prognosis.

OBJECTIVE

The intention of the examine is to guage the function of Denaturing Excessive Efficiency Liquid Chromatography (DHPLC) within the second degree screening of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene.

METHODS

A 9-month potential examine, between June 2008 and March 2009 at Artemisia Fetal Medical Centre, included 3829 samples of amniotic fluid collected from girls present process mid-trimester amniocentesis.The genetic prognosis of CF was primarily based on analysis of the principle mutations of the CFTR gene on fetal DNA extracted from the amniocytes, (first degree screening) utilizing completely different business diagnostic programs. A second degree screening utilizing DHPLC, on the amniotic fluid and on a blood pattern from the couple, was supplied in case of fetuses heterozygous at first degree screening.

RESULTS

Of 3829 fetuses, 134 had been discovered to be optimistic, 129 heterozygous and 5 affected. Of the 129 {couples}, following acceptable genetic counselling, 53 requested a second degree screening. By means of using DHPLC, 44 {couples} had been discovered to be damaging, and in 9 {couples}, 9 uncommon mutations had been recognized.

CONCLUSIONS

The primary degree screening will be helpful to proof as much as 75% of the CF mutations. The second degree screening can establish an additional 10% of mutant alleles. DHPLC was discovered to be a dependable and particular technique for the speedy identification of the uncommon CFTR mutations which weren’t revealed in preliminary first degree screening.

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