DUA Gene Mutation Detection with Combined Use of dHPLC

sanyo-biomedical

Environment friendly IDUA Gene Mutation Detection with Mixed Use of dHPLC and Dried Blood Samples.

Goals. Improvement of a easy mutation directed methodology with the intention to enable decreasing the price of mutation testing utilizing an simply obtainable organic materials. Evaluation of the feasibility of such methodology was examined utilizing a GC-rich amplicon. Design and Strategies.

A way of denaturing high-performance liquid chromatography (dHPLC) was improved and carried out as a way for the detection of variants in exon 9 of the IDUA gene. The optimized methodology was examined in 500 genomic DNA samples obtained from dried blood spots (DBS).

Outcomes. With this dHPLC strategy it was attainable to detect completely different variants, together with the frequent p.Trp402Ter mutation within the IDUA gene. The excessive GC content material didn’t intrude with the decision and reliability of this system, and discrimination of G-C transversions was additionally achieved. Conclusion.

This PCR-based dHPLC methodology is proved to be a fast, a delicate, and a very good choice for screening quite a few samples obtained from DBS. Moreover, it resulted within the constant detection of clearly distinguishable profiles of the frequent p.Trp402Ter IDUA mutation with an advantageous steadiness of value and technical necessities.

Using COLD-PCR, DHPLC and GeneScanning for the extremely delicate detection of c-KIT somatic mutations in canine mast cell tumours.

The traditional polymerase chain response (PCR)/sequencing strategies could also be poorly fitted to the detection of somatic mutations in canine mast cell tumour (MCT) samples owing to restricted sensitivity. This research was aimed toward establishing novel and extra delicate strategies, assessing their restrict of detection and evaluating their sensitivity with typical strategies.Two completely different ‘driver’ somatic mutations of c-KIT, along with the wild-type counterparts, have been cloned in plasmids to organize normal samples with identified concentrations of mutated alleles in a background of wild-type alleles; the plasmids requirements have been assayed utilizing both typical or novel, extremely delicate approach.

Standard PCR/sequencing confirmed a sensitivity of 50-20%. Conversely, all of the novel strategies obtained larger sensitivities allowed reaching as little as 2.5-1.2% of the mutated DNA.The research demonstrates that early typical strategies might doubtless have underestimated the prevalence of KIT mutations of MCTs, due to this fact affecting the evaluation of their relevance in prognosis and tyrosine kinase inhibitor (TKI) therapy effectiveness.

sanyo-biomedical
sanyo-biomedical

Mouse Filaggrin (FLG) ELISA Kit

RDR-FLG-Mu-48Tests 48 Tests
EUR 557

Mouse Filaggrin (FLG) ELISA Kit

RDR-FLG-Mu-96Tests 96 Tests
EUR 774

Mouse Filaggrin (FLG) ELISA Kit

RD-FLG-Mu-48Tests 48 Tests
EUR 533

Mouse Filaggrin (FLG) ELISA Kit

RD-FLG-Mu-96Tests 96 Tests
EUR 740

Recombinant Filaggrin (FLG)

4-RPJ103Hu01
  • EUR 816.80
  • EUR 322.00
  • EUR 2788.00
  • EUR 996.00
  • EUR 1892.00
  • EUR 610.00
  • EUR 6820.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Filaggrin expressed in: E.coli

Recombinant Filaggrin (FLG)

4-RPJ103Mu01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Filaggrin expressed in: E.coli

Human Filaggrin (FLG)

1-CSB-YP008712HU
  • EUR 430.00
  • EUR 234.00
  • EUR 1508.00
  • EUR 642.00
  • EUR 1009.00
  • EUR 291.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Filaggrin(FLG),partial expressed in Yeast

Human Filaggrin (FLG)

1-CSB-EP008712HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Filaggrin(FLG),partial expressed in E.coli

Human Filaggrin (FLG)

1-CSB-BP008712HU
  • EUR 840.00
  • EUR 332.00
  • EUR 2170.00
  • EUR 1135.00
  • EUR 1579.00
  • EUR 470.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Filaggrin(FLG) ,partial expressed in Baculovirus

Filaggrin (FLG) Antibody

20-abx133148
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Filaggrin (FLG) Antibody

20-abx109789
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Filaggrin (FLG) Antibody

20-abx100249
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody

20-abx100250
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody

20-abx172440
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug

Filaggrin (FLG) Antibody

20-abx339766
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Filaggrin (FLG) Antibody

20-abx339302
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Filaggrin (FLG) Antibody

20-abx176473
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Human Filaggrin (FLG) Protein

20-abx066657
  • EUR 1121.00
  • EUR 411.00
  • EUR 3724.00
  • EUR 1358.00
  • EUR 759.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Filaggrin (FLG) ELISA Kit

20-abx151555
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Filaggrin (FLG) ELISA Kit

abx572526-96tests 96 tests
EUR 668

Human FLG/ Filaggrin ELISA Kit

E0920Hu 1 Kit
EUR 571

Human Filaggrin (FLG)ELISA Kit

201-12-2700 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Filaggrin (FLG) CLIA Kit

20-abx190272
  • EUR 7911.00
  • EUR 4215.00
  • EUR 973.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Filaggrin ELISA Kit (FLG)

RK01403 96 Tests
EUR 521

Human Filaggrin(FLG) ELISA kit

CSB-EL008712HU-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human Filaggrin (FLG) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Filaggrin(FLG) ELISA kit

1-CSB-EL008712HU
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Filaggrin(FLG) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Filaggrin (FLG)CLIA Kit

SCJ103Hu-10x96wellstestplate 10x96-wells test plate
EUR 5647.8
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG)CLIA Kit

SCJ103Hu-1x48wellstestplate 1x48-wells test plate
EUR 552.76
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG)CLIA Kit

SCJ103Hu-1x96wellstestplate 1x96-wells test plate
EUR 746.8
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG)CLIA Kit

SCJ103Hu-5x96wellstestplate 5x96-wells test plate
EUR 3060.6
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG) CLIA Kit

4-SCJ103Hu
  • EUR 5698.00
  • EUR 3061.00
  • EUR 747.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Filaggrin (FLG)Tissue homogenates and other biological fluids

Filaggrin (FLG) Polyclonal Antibody (Human)

4-PAJ103Hu01
  • EUR 247.00
  • EUR 2510.00
  • EUR 625.00
  • EUR 310.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG)

Human Filaggrin (FLG) ELISA Kit

SEJ103Hu-10x96wellstestplate 10x96-wells test plate
EUR 4731.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit

SEJ103Hu-1x48wellstestplate 1x48-wells test plate
EUR 477.3
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit

SEJ103Hu-1x96wellstestplate 1x96-wells test plate
EUR 639
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit

SEJ103Hu-5x96wellstestplate 5x96-wells test plate
EUR 2575.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit

4-SEJ103Hu
  • EUR 4782.00
  • EUR 2526.00
  • EUR 640.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Filaggrin (FLG) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Filaggrin(FLG)ELISA Kit

QY-E01412 96T
EUR 374

Human FLG(Filaggrin) ELISA Kit

EH1353 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human Filaggrin, FLG ELISA KIT

ELI-03920h 96 Tests
EUR 824

Anti-Filaggrin/FLG Antibody

PB9870 100ug/vial
EUR 334

Filaggrin (FLG) Antibody (FITC)

20-abx106841
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Filaggrin (FLG) Antibody (HRP)

20-abx108261
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Filaggrin (FLG) Antibody (Biotin)

20-abx105422
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Mouse Filaggrin (FLG) Protein

20-abx066658
  • EUR 732.00
  • EUR 286.00
  • EUR 2305.00
  • EUR 885.00
  • EUR 523.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody (FITC)

20-abx271155
  • EUR 495.00
  • EUR 258.00
  • EUR 1455.00
  • EUR 676.00
  • EUR 398.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody (Biotin)

20-abx271421
  • EUR 467.00
  • EUR 244.00
  • EUR 1344.00
  • EUR 634.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody (Biotin)

20-abx272419
  • EUR 453.00
  • EUR 244.00
  • EUR 1316.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Filaggrin (FLG) Antibody (FITC)

20-abx273568
  • EUR 481.00
  • EUR 244.00
  • EUR 1414.00
  • EUR 662.00
  • EUR 356.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

ELISA kit for Human FLG (Filaggrin)

ELK3513 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Filaggrin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Filaggrin (FLG) Polyclonal Antibody (Human), APC

4-PAJ103Hu01-APC
  • EUR 345.00
  • EUR 3275.00
  • EUR 912.00
  • EUR 440.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with APC.

Filaggrin (FLG) Polyclonal Antibody (Human), Biotinylated

4-PAJ103Hu01-Biotin
  • EUR 311.00
  • EUR 2460.00
  • EUR 727.00
  • EUR 381.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with Biotin.

Filaggrin (FLG) Polyclonal Antibody (Human), Cy3

4-PAJ103Hu01-Cy3
  • EUR 419.00
  • EUR 4325.00
  • EUR 1175.00
  • EUR 545.00
  • EUR 251.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with Cy3.

Filaggrin (FLG) Polyclonal Antibody (Human), FITC

4-PAJ103Hu01-FITC
  • EUR 296.00
  • EUR 2640.00
  • EUR 750.00
  • EUR 372.00
  • EUR 195.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with FITC.

Filaggrin (FLG) Polyclonal Antibody (Human), HRP

4-PAJ103Hu01-HRP
  • EUR 316.00
  • EUR 2855.00
  • EUR 807.00
  • EUR 398.00
  • EUR 206.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with HRP.

Filaggrin (FLG) Polyclonal Antibody (Human), PE

4-PAJ103Hu01-PE
  • EUR 296.00
  • EUR 2640.00
  • EUR 750.00
  • EUR 372.00
  • EUR 195.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with PE.

Mouse Filaggrin (FLG) ELISA Kit

20-abx154027
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Filaggrin (FLG) ELISA Kit

abx573034-96tests 96 tests
EUR 668

Mouse Filaggrin (FLG) CLIA Kit

20-abx495617
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Flg/ Filaggrin ELISA Kit

E0543Mo 1 Kit
EUR 571

Mouse Filaggrin (FLG) ELISA Kit

abx257768-96tests 96 tests
EUR 746

Mouse Filaggrin ELISA Kit (FLG)

RK02813 96 Tests
EUR 521

Mouse FLG(Filaggrin) ELISA Kit

EM1853 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 9.375pg/ml

Mouse Filaggrin(FLG) ELISA kit

CSB-EL008712MO-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Mouse Filaggrin (FLG) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Filaggrin(FLG) ELISA kit

1-CSB-EL008712MO
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse Filaggrin(FLG) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Filaggrin (FLG) ELISA Kit

SEJ103Mu-10x96wellstestplate 10x96-wells test plate
EUR 4862.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

SEJ103Mu-1x48wellstestplate 1x48-wells test plate
EUR 488.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

SEJ103Mu-1x96wellstestplate 1x96-wells test plate
EUR 654.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

SEJ103Mu-5x96wellstestplate 5x96-wells test plate
EUR 2644.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit

4-SEJ103Mu
  • EUR 4913.00
  • EUR 2595.00
  • EUR 655.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Filaggrin (FLG) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Filaggrin(FLG)ELISA Kit

QY-E21727 96T
EUR 374

Mouse Filaggrin, Flg ELISA KIT

ELI-03919m 96 Tests
EUR 865

Filaggrin (FLG) Polyclonal Antibody (Human), APC-Cy7

4-PAJ103Hu01-APC-Cy7
  • EUR 571.00
  • EUR 6430.00
  • EUR 1705.00
  • EUR 760.00
  • EUR 319.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with APC-Cy7.

ELISA kit for Mouse FLG (Filaggrin)

ELK6992 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Filaggrin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat)

4-PAJ103Mu01
  • EUR 251.00
  • EUR 2576.00
  • EUR 640.00
  • EUR 316.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG)

ELISA kit for Human FLG-Ab (Anti-Filaggrin)

E-EL-H0283 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human FLG-Ab (Anti-Filaggrin) in samples from Serum, Plasma, Cell supernatant

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561) Antibody

BNC941561-100 100uL
EUR 199
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561),CF594 conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561) Antibody

BNC941561-500 500uL
EUR 544
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561),CF594 conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562) Antibody

BNC941562-100 100uL
EUR 199
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562),CF594 conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562) Antibody

BNC941562-500 500uL
EUR 544
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562),CF594 conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563) Antibody

BNC941563-100 100uL
EUR 233
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563), CF594 conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563) Antibody

BNC941563-500 500uL
EUR 545
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563), CF594 conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945) Antibody

BNC941945-100 100uL
EUR 233
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945), CF594 conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945) Antibody

BNC941945-500 500uL
EUR 545
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945), CF594 conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R) Antibody

BNC941957-100 100uL
EUR 233
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R), CF594 conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R) Antibody

BNC941957-500 500uL
EUR 545
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R), CF594 conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561) Antibody

BNCA1561-250 250uL
EUR 383
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561),APC conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562) Antibody

BNCA1562-250 250uL
EUR 383
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562),APC conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563) Antibody

BNCA1563-250 250uL
EUR 394
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563), APC conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945) Antibody

BNCA1945-250 250uL
EUR 394
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945), APC conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R) Antibody

BNCA1957-250 250uL
EUR 394
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R), APC conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561) Antibody

BNC811561-100 100uL
EUR 199
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561),CF680R conjugate, Concentration: 0.1mg/mL

 

Use of denaturing high-performance liquid chromatography (DHPLC) to characterize the bacterial and fungal airway microbiota of cystic fibrosis sufferers. 

The goal of this research was to guage using denaturing high-performance liquid chromatography (DHPLC) to characterize cystic fibrosis (CF) airway microbiota together with each micro organism and fungi. DHPLC situations have been first optimized utilizing a combination of V6, V7 and V8 area 16S rRNA gene PCR amplicons from 18 bacterial species generally present in CF sufferers. Then, the microbial range of four sputum samples from four CF sufferers was analyzed utilizing cultural strategies, cloning/sequencing (for micro organism solely) and DHPLC peak fraction assortment/sequencing. DHPLC evaluation allowed figuring out extra bacterial and fungal species than the classical tradition strategies, together with well-recognized pathogens corresponding to Pseudomonas aeruginosa.

Even when a decrease variety of bacterial Operational Taxonomic Items (OTUs) was recognized by DHPLC, it allowed to seek out OTUs unidentified by cloning/sequencing. The mixture of each strategies permitted to correlate nearly all of DHPLC peaks to outlined OTUs. Lastly, though Aspergillus fumigatus detection utilizing DHPLC can nonetheless be improved, this system clearly allowed to establish a better variety of fungal species versus classical culture-based strategies. To conclude, DHPLC offered significant extra information regarding pathogenic micro organism and fungi in addition to fastidious microorganisms current inside the CF respiratory tract. DHPLC will be thought-about as a complementary approach to culture-dependent analyses in routine microbiological laboratories.

DHPLC and MS research of a photoinduced intrastrand cross-link in DNA labeled with 5-bromo-2′-deoxyuridine

It’s well-known that the alternative of thymidine with 5-bromo-2′-deoxyuridine (BrdU) in DNA sensitizes it to UVB gentle. Irradiation of a biopolymer substituted in such a manner results in manifold sorts of DNA harm, corresponding to intrastrand cross-links, single- and double-strand breaks or alkali-labile websites that have been studied previously with a broad spectrum of analytical strategies. Right here, we show that fully denaturing high-performance liquid chromatography (DHPLC), underestimated to date in DNA harm research, might act as a cheap, and high-resolution substitute for the generally employed gel electrophoresis.

We report on the DHPLC/mass spectrometry (MS) analyses of photolytes obtained with the UV irradiation of aqueous options containing 40 base pairs of a protracted, double-stranded oligonucleotide labeled with BrdU in one among its strands. The UV-product was detected by HPLC at a temperature of 70°C. Subsequent MS evaluation with electrospray ionization (ESI-MS) of the photolyte, enzymatic digestion of the irradiated materials and HPLC and MS evaluation (LC-MS) of the digest demonstrated unequivocally that an intrastrand covalent dimer, involving adenine and uracil, is shaped within the irradiated system.

 

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